Antitumor vector systems based on bioactive lectin of Bacillus subtilis ІМВ B-7724

Сombination of properties lectins and magnetically sensitive iron-containing nanocomposites (NC) for use in oncology is actual promising from scientific applied point view. The aim the research to synthesize study new NC magnetic fluids containing bioactive bacterial lectin, as prototypes effective antitumor vector systems targeted drug delivery combined local therapy cancer with minimized side effects on body improved compatibility other remedies. To create systems, nanodisperse magnetite was synthesized by Elmore reaction. synthesis aluminum-containing coating surface Fe3O4 carried out double chemical modification aluminum isopropylate. obtained Fe3O4/Al2O3 impregnated sucrose solutions. Carbonization carbohydrate shell argon (500 °C). As a result, Fe3O4/Al2O3/C obtained. nanostructures were measured using laboratory vibration magnetometer Foner type at room temperature. Adsorption immobilization lectin performed 0.9 % NaCl solution dynamic mode Bacterial cytotoxic B. subtilis IMB B-7724 used experiments. amount adsorbed substance (A) determined measuring concentration contact solutions before after adsorption calibration graph. Measuring optical density absorption spectra spectrometer Lambda 35 UV/vis Perkin Elmer Instruments λ = 280 nm. Standard techniques equipment biological research. processes physiologic saline carbon-containing studied It has been found that capacity 25.3 mg/g, – 36.3 mg/g (at initial concentrations 0.06–0.4 mg/mL). extraction extent R (%) 12–38 46–67 NC. dependence time studied. A fluid (MF) based single-domain Fe3O4, investigated. Immobilization MF particles temperature 3 hours. composition 0.2 mg/mL. immobilized further modified PEG-2000. Fe3O4/ol.Na/lectin/PEG (ol.Na sodium oleate) system Modification nanoparticles polyethylene glycol order increase stability fluid, reducing aggregation particles. determine effect experimental samples viability MCF-7 cells vitro, following prepared: Fe3O4/ol.Na/PEG (MF), CFe3O4 mg/mL; (CL), CCL nanobiocomposite (NBC). Nanobiocomposite have synergistic human breast cells, causing up 40 cell death. IC50 values relation 100 125 μg/mL, respectively. results show combination direction creating cancer. natural components way minimize improve